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Product Info Summary
| SKU: | LS025797 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of glutaminyl-peptide cyclotransferase (QPCT) |
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Product info
Product Name
QPCT (NM_012413) Human Over-expression Lysate
See all Glutaminyl-peptide Cyclotransferase/QPCT products
SKU/Catalog Number
LS025797
Size
100 µg
Description
Transient overexpression lysate of glutaminyl-peptide cyclotransferase (QPCT)
Storage & Handling
Cite This Product
QPCT (NM_012413) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS025797)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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QPCT Human Over-expression Lysates NM_012413.
Western blot validation of overexpression lysate (LS025797) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with QPCT (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for QPCT (Source: Uniprot.org, NCBI)
Gene Name
QPCT
Full Name
Glutaminyl-peptide cyclotransferase
Weight
40.877kDa
Superfamily
glutaminyl-peptide cyclotransferase family
Alternative Names
EC 2.3.2.5; GCT; Glutaminyl cyclase; glutaminyl-peptide cyclotransferase; Glutaminyl-tRNA cyclotransferase; Glutamyl cyclase; QCEC; QPCT; sQC QPCT GCT, QC, sQC glutaminyl-peptide cyclotransferase glutaminyl-peptide cyclotransferase|EC|glutaminyl cyclase|glutaminyl-tRNA cyclotransferase|glutamyl cyclase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on QPCT, check out the QPCT Infographic
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In this infographic, you will see the following information for QPCT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For QPCT (NM_012413) Human Over-expression Lysate (LS025797)
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No publications found for LS025797
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